Target discovery in biological systems relies on access to relevant genetic diversity. Historically, genetic diversity has been sourced through natural variation, classical mutagenesis approaches, or more recently using molecular genetic tools such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases and CRISPR nucleases to deliver targeted genetic manipulations. While target discovery has been performed using each of these approaches, the ability to generate large populations of cells with precisely targeted and controlled genetic variation throughout the entire genome has never been an option in this field of study.
Inscripta’s Onyx platform now makes this possible.
Onyx allows you to design your experiments, generate thousands of unique edited cells, assess and test your library quality, interrogate genotypes in your engineered cell populations, and rapidly analyze and identify novel genotype-phenotype associations.
The scope of discovery that once took years to decades to achieve can now be accomplished in a matter of months to years. With rapid genome engineering and genome-wide CRISPR library screening you can create and test more ideas, and discover and exploit new biology in areas such as biomass hydrolysate component growth inhibition, antibiotic resistance, and adaptive laboratory evolution (ALE), for rapid target discovery.
Microbial genome engineering workflow using the Onyx benchtop platform:
Design: edit library is designed using InscriptaDesigner™ software.
Engineer cells: cells are engineered using Onyx system and Onyx genome engineering chemistry.
Genotype: cells are genotyped using Onyx assays.
Analyze and learn: results are analyzed using InscriptaResolver™ software.